Super-resolution imaging of subcortical white matter using stochastic optical reconstruction microscopy (STORM) and super-resolution optical fluctuation imaging (SOFI)
نویسندگان
چکیده
منابع مشابه
Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI).
Super-resolution optical microscopy is a rapidly evolving area of fluorescence microscopy with a tremendous potential for impacting many fields of science. Several super-resolution methods have been developed over the last decade, all capable of overcoming the fundamental diffraction limit of light. We present here an approach for obtaining subdiffraction limit optical resolution in all three d...
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Recent advances in far-field fluorescence microscopy have led to substantial improvements in image resolution, achieving a near-molecular resolution of 20 to 30 nanometers in the two lateral dimensions. Three-dimensional (3D) nanoscale-resolution imaging, however, remains a challenge. We demonstrated 3D stochastic optical reconstruction microscopy (STORM) by using optical astigmatism to determi...
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Super-resolution optical fluctuation imaging (SOFI) allows one to perform sub-diffraction fluorescence microscopy of living cells. By analyzing the acquired image sequence with an advanced correlation method, i.e. a high-order cross-cumulant analysis, super-resolution in all three spatial dimensions can be achieved. Here we introduce a software tool for a simple qualitative comparison of SOFI i...
متن کاملLive-cell multiplane three-dimensional super-resolution optical fluctuation imaging
Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefield microscope. Previously, three-dimensional (3D) SOFI has been demonstrated by sequential imaging of multiple depth positions. Here we intr...
متن کاملEasy Ways to High- and Super-Resolution Fluorescence Microscopy: Image Scanning Microscopy (ISM), and Stochastic Optical Fluctuation Imaging (SOFI), and Metal-Induced Energy Transfer (MIET)
Classical fluorescence microscopy is limited in resolution by the wavelength of light (diffraction limit) restricting lateral resolution to ca. 200 nm, and axial resolution to ca. 500 nm (at typical excitation and emission wavelengths around 500 nm). However, recent years have seen a tremendous development in highand super-resolution techniques of fluorescence microscopy, pushing spatial resolu...
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ژورنال
عنوان ژورنال: Neuropathology and Applied Neurobiology
سال: 2017
ISSN: 0305-1846
DOI: 10.1111/nan.12426